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In order to investigate potential residual confounding by population stratification, we adjusted for principal components derived from IBC CardioChip array 24 in studies with rapid data see supplementary methods 2. To evaluate confounding by development disequilibrium between rs with single nucleotide polymorphisms associated with cardiovascular risk factors in genome-wide association studies 29 that could distort associations of rs, we used the Whitehall-II cohort 30 including both IBC CardioChip 24 and MetaboChip 31 platforms.
In the current manuscript, we did not conduct an instrumental variable analysis to estimate paper effects since the available researches assume a linear association between the exposure and the outcome, which may not hold for application and cardiovascular disease.
Analyses were conducted in Stata v All P values reported are two sided.
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Results We approached 59 studies, of which 56 were included in this collaboration. The median number of alcohol units consumed in each study is shown in table S4. There were 20 coronary heart disease events, 10 stroke cases ischaemic strokes and 14 type 2 diabetes cases table S5. Means and distributions for continuous traits in all studies are presented in tables S6-S8.
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The observational analysis between alcohol and cardiovascular risk factors is reported in the supplementary results section and figures S2 and S3 of the appendix. Rs A-allele carriers also had lower odds of binge drinking odds ratio 0. A meta-regression analysis of the mean alcohol volume on the log scale in rs A-allele carriers compared with non-carriers that takes into account the uncertainty around the mean suggested a constant proportional effect of the of ADH1B rs variant on alcohol volume fig S5.
This was also supported by the finding that in our samples the standard deviations for carriers and non-carriers were very similar fig S6. Table 1 Pooled estimates of association between genetic variant ADH1B rs A-allele carriers v non-carriers and measures of alcohol consumption. Summary effect estimates are derived from fixed effects meta-analysis View this table: Concordant with this, rs A-allele carriers also had lower odds of hypertension cases; odds ratio be resume format.
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Table 2 Pooled estimates of association between genetic variant ADH1B rs A-allele carriers v non-carriers and year 7 science homework booklet answers biomarkers in all participants. Summary effect estimates are derived from fixed effects meta-analysis and are reported as mean differences unless stated otherwise View this table: View inline When the effect of the ADH1B rs A-allele on these cardiovascular traits was stratified by alcohol consumption, a differential effect was observed.
Fig 1 Meta-analysis pooled estimates of the association between ADH1B rs A-allele carriers v non-carriers and cardiovascular disease biomarkers showing association on crude analysis, stratified by alcohol intake.
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